Peer-reviewed veterinary case report
Rapid development of polyclonal antisera against infectious salmon anaemia virus and its optimization and application as a diagnostic tool.
- Journal:
- Journal of fish diseases
- Year:
- 2006
- Authors:
- McBeath, S J et al.
- Affiliation:
- FRS Marine Laboratory · United Kingdom
Abstract
Infectious salmon anaemia is an important disease of Atlantic salmon. One of the current methods of diagnosis is the indirect fluorescent antibody test (IFAT), using a monoclonal antibody specific to the haemagglutinin of the virus. The conformationally dependent nature of this antibody could be a drawback in its usefulness in other tests. This study describes the development and optimization of a polyclonal antiserum against infectious salmon anaemia virus, including a method of separating virus from cell culture components within culture supernatant. The antiserum was subsequently optimized for use in a variety of immunological diagnostic tests, including IFAT and an alkaline phosphatase-based immunoassay, and Western blot.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/16677319/