Peer-reviewed veterinary case report
Fast test to detect four dog respiratory viruses with PCR
By Zhou, Hu et al.·Published in Journal of virological methods·2024·Huazhong Agricultural University, China·View original on PubMed →
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Original publication title: Rapid diagnosis of canine respiratory coronavirus, canine influenza virus, canine distemper virus and canine parainfluenza virus with a Taqman probe-based multiplex real-time PCR.
- Species:
- dog
Plain-English summary
A group of dogs with respiratory symptoms, such as coughing and difficulty breathing, were tested for several contagious viruses that can cause similar issues. Researchers developed a new test that can quickly identify four specific viruses: canine respiratory coronavirus, canine influenza virus, canine distemper virus, and canine parainfluenza virus. This test is more efficient than older methods, allowing vets to get results faster and more accurately. By using this new test, veterinarians can better diagnose and manage respiratory illnesses in dogs, helping them recover more effectively.
People also search for: dog coughing treatment · canine influenza virus symptoms · how to test for dog respiratory viruses
Abstract
Canine Infectious Respiratory Disease Complex (CIRDC) is a highly infectious diseases. Canine respiratory coronavirus (CRCoV), Canine influenza virus (CIV), Canine distemper virus (CDV), and Canine parainfluenza virus (CPiV) are crucial pathogens causing CIRDC. Due to the similar clinical symptoms induced by these viruses, differential diagnosis based solely on symptoms can be challenging. In this study, a multiplex real-time PCR assay was developed for detecting the four RNA viruses of CIRDC. Specific primers and probes were designed to target M gene of CRCoV, M gene of CIV, N gene of CDV and NP gene of CPiV. The detection limit is 10 copies/μL for CIV or CRCoV, while the detection limit of CDV or CPiV is 100 copies/μL. Intra-group and inter-group repeatability coefficient of variation (CV) were both less than 2 %. A total of 341 clinical canine samples were analyzed, and the results indicated that the method developed in our study owns a good consistency and better specificity compared with the conventional reverse transcription PCR. This study provides a new method to enable the simultaneous detection of all four pathogens in a single reaction, improving the efficiency for monitoring the prevalence of four viruses in CIRDC, which benefits the control of CIRDC.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/38823586/