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Peer-reviewed veterinary case report

Rapid diagnosis of Vibrio owensii responsible for shrimp acute hepatopancreatic necrosis disease with isothermal recombinase polymerase amplification assay.

Journal:
Molecular and cellular probes
Year:
2017
Authors:
Liu, Liyuan et al.
Affiliation:
College of Food Science and Technology · China

Abstract

A rapid and sensitive AHPND-RPA assay was developed for the specific detection of the AHPND-causing Vibrio owensii. The AHPND-RPA detected as few as 2 copies per reaction in 9.02 ± 0.66 min at 39 °C, and showed 100% positive predictive value and negative predictive value for AHPND diagnosis.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/28179103/