Recombinant MS087-based indirect ELISA for the diagnosis of.

Abstract

Accurate detection is a prerequisite for effective prevention and control ofinfection. ELISA is the most popular method for the clinical detection ofbecause of its convenience, low cost, and high detection rate. However, the cross-reactivity of commercially available ELISA kits with other avian pathogen-positive sera needs to be addressed. The aim of this study was to establish an ELISA method with high specificity for the detection of anti-antibodies in chicken serum to evaluate theinfection status on poultry farms. The recombinant MS087 (rMS087) protein was expressed inBL21 (DE3) and purified by Niaffinity chromatography. An antibody against rMS087 was generated by immunizing BALB/c mice. Bioinformatic analysis revealed that MS087 was conserved amongstrains. Western blotting and indirect immunofluorescence results indicated that MS087 was not only localized in the cytoplasm and on the membrane but also secreted by the organism. For the established ELISA method based on rMS087, the optimal antigen concentration, blocking buffer, blocking duration, serum dilution, serum incubation duration, secondary antibody dilution, secondary antibody incubation duration and colorimetric reaction duration were 2 μg/mL, 1% BSA, 3 h, 1:500, 1.5 h, 1:20,000, 2 h and 5 min, respectively. Validation of the rMS087-based ELISA revealed a cut-off value of 0.5. The coefficients of variation of both the intra-batch and inter-batch methods were less than 9%. The assay was able to differentiate positive serum againstfrom antisera against nine other avian pathogens and was able to recognize-positive sera at a dilution of 1:1,000. Compared with the commercial ELISA method, the rMS087-based ELISA has the potential to recognize more positive sera against. Collectively, the rMS087-based ELISA is a reproducible, specific, and sensitive serological method for detecting antibodies againstin chicken serum and has robust potential for large-scale serological epidemiology ofinfection on poultry farms.