Peer-reviewed veterinary case report
Using recombinant OmpL1 protein to diagnose leptospirosis in dogs
By Subathra, M et al.·Published in Applied biochemistry and biotechnology·2013·Department of Animal Biotechnology, India·View original on PubMed →
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Original publication title: Recombinant OmpL1 protein as a diagnostic antigen for the detection of canine leptospirosis.
- Species:
- dog
Plain-English summary
A study found that a new blood test using a specific protein (OmpL1) can help diagnose leptospirosis in dogs more effectively than the traditional method. In testing 475 dog blood samples, the new test identified 294 cases of leptospirosis, while the older method only detected 283. The new test was able to catch some cases that the older method missed, showing it could be more reliable. This means that if your dog is showing symptoms of leptospirosis, like fever or vomiting, this new test could provide a more accurate diagnosis.
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Abstract
Microscopic agglutination test (MAT) is the standard method for the diagnosis of leptospirosis, which is laborious and the interpretation of the results is subjective. The present work describes the use of recombinant-based IgG ELISA for the serodiagnosis of leptospirosis. We used recombinant outer membrane protein OmpL1 as an antigen for conducting IgG enzyme-linked immunosorbent assay (ELISA). A total of 475 canine serum samples were subjected to IgG ELISA; 294 sera were positive to ELISA, while 283 were positive to MAT. All samples that were positive to MAT were positive to ELISA also, however, few samples which were negative to MAT were positive to ELISA, which suggested that recombinant-based IgG ELISA showed 100 % sensitivity when compared to MAT. Thus, this present study showed that recombinant OmpL1-based IgG ELISA appears to be a better alternative to MAT for the diagnosis of leptospirosis and rOmpL1 protein could be used as a potential diagnostic antigen in different assay formats for leptospirosis.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/23212848/