Regulation of Second Basal Internode Characteristics by Nitrogen Fertilizer Enhances Lodging Resistance and Yield in Winter Wheat (<i>Triticum aestivum</i> L.).

Abstract

In the North China Plain (NCP), wind and rain during the grain-filling period of winter wheat can cause lodging. The second basal internode (I2), a key load-bearing structure, plays a central role in yield stability. This study, under a constant nitrogen (N) application rate of 270 kg ha^-1, aimed to clarify how nitrogen basal-to-topdressing ratios regulate I2 characteristics to balance lodging resistance and yield increase. Field experiments were conducted across two seasons with three cultivars and three nitrogen split ratios (5:5, CK; 3:7, N1; and 7:3, N2). Dynamic measurements of I2 mechanical properties, morphology, anatomy, and composition were taken, and structural equation modeling (SEM) was used for analysis. Results showed that the culm lodging resistance index (CLRI) decreased by 41.8% from flowering to milk stage under all treatments, with CLRI at the milk stage of lodging treatments between 0.11 and 0.15. SEM supported a composition-structure-lodging resistance-yield chain, with CLRI as the key mediator. The N1 treatment significantly improved CLRI at all stages and increased yield by 12.2% compared to CK, making it a recommended nitrogen strategy for improving both yield and lodging resistance. These findings provide agronomically applicable nitrogen management guidelines for high-yield winter wheat systems.