Remote preconditioning reduces microcirculatory disorders in pancreatic ischemia/reperfusion injury.

Abstract

OBJECTIVES: Remote preconditioning (RPC) can protect from ischemia/reperfusion injury (IRI). We investigated the influence of RPC in pancreatic IRI. METHODS: Wistar rats were randomized to 2 hours of ischemia and 2 hours of reperfusion of a pancreatic tail segment with or without 15 minutes of infrarenal ischemia 60 minutes before IRI. Microcirculatory measurements before ischemia and 1 and 2 hours after reperfusion included functional capillary density and leukocyte adherence in postcapillary venules, quantified by intravital fluorescence microscopy. Histology and tissue myeloperoxidase activity were further parameters of pancreatic injury. RESULTS: Remote preconditioning caused an improvement of microcirculation (functional capillary density: 1 hour after reperfusion, 460 +/- 13 vs 350 +/- 9 cm/cm2; 2 hours after reperfusion, 437 +/- 13 vs 295 +/- 13 cm/cm2; P < 0.01) and reduced inflammatory tissue response (leukocyte adherence in postcapillary venules: 2 hours after reperfusion, 155 +/- 55 vs 748 +/- 187 cells/mm2; P < 0.01). Histology was significantly better in preconditioned animals (IR, 8.1+/- 1.3 score points; RPC, 6.2 +/- 1.3 score points; P < 0.05). The difference in myeloperoxidase activity was not significant (ischemia/reperfusion [IR], 105 +/- 72; RPC, 245 +/- 209 mU x min(-1) x mg(ti)(-1); P = 0.13). CONCLUSIONS: With our dynamic functional microcirculatory measurements, we could demonstrate that RPC is a feasible method to reduce experimental pancreatic IRI. This was seen in an attenuation of nutritive tissue perfusion and a reduction of inflammatory tissue response and a lower histological damage. Because it is easy to perform before organ harvest, RPC could be a step to improve organ procurement in pancreas transplantation. Clinical studies are the next step to evaluate RPC in pancreas transplantation.