Renal acid excretion contributes to acid-base regulation during hypercapnia in air-exposed swamp eel ().

Abstract

The swamp eel () uses its buccal cavity to air breathe, while the gills are strongly reduced. It burrows into mud during the dry season, is highly tolerant of air exposure, and experiences severe hypoxia both in its natural habitat and in aquaculture. To study the ability ofto compensate for respiratory acidosis, we implanted catheters to sample both arterial blood and urine during hypercapnia (4% CO) in either water or air, or during whole-animal air exposure. These hypercapnic challenges caused an immediate reduction in arterial pH, followed by progressive compensation through a marked elevation of plasma HCOover the course of 72 h. There was no appreciable rise in urinary acid excretion in fish exposed to hypercapnia in water, although urine pH was reduced and ammonia excretion did increase. In the air-exposed fish, however, hypercapnia was attended by a large elevation of ammonia in the urine and a large rise in titratable acid excretion. The time course of the increased renal acid excretion overlapped with the time period required to elevate plasma HCO, and we estimate that the renal compensation contributed significantly to whole-body acid-base compensation.