Peer-reviewed veterinary case report
Renal artery injection for delivery of biological materials to the glomerulus.
- Journal:
- Nephrology (Carlton, Vic.)
- Year:
- 2008
- Authors:
- Takabatake, Yoshitsugu et al.
- Affiliation:
- Department of Nephrology · Japan
- Species:
- rodent
Abstract
AIM: We investigated whether injection of synthetic small interfering siRNAs via renal artery followed by electroporation could be effective in silencing specific genes in glomerulus. METHODS: Solution of siRNA targeting enhanced green fluorescent protein (EGFP) was injected via the renal artery of EGFP-transgenic rats followed by delivery of electric pulses. RESULTS: The delivery of siRNA reduced endogenous EGFP expression, mainly in glomerular mesangial cells. CONCLUSION: Electroporation-mediated gene transfer system via renal artery in rats is very useful for the delivery of a range of different materials including cells, viruses and proteins. Here, we provide a step-by-step description of this perfusion method and a discussion of the key points for the success of the technique.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/18199097/