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Peer-reviewed veterinary case report

How reliable is ear cytology for diagnosing dog ear infections

By Lehner, G et al.·Published in The Veterinary record·2010·Small Animal Medicine Clinic, Germany·View original on PubMed

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Original publication title: Reproducibility of ear cytology in dogs with otitis externa.

Species:
dog

Plain-English summary

A group of 83 dogs, including Golden Retrievers and West Highland White Terriers, showed signs of ear infections (otitis externa) and had microbial organisms found in their ear samples. The study involved taking swabs from both ears to check for bacteria and yeast. It was noted that dogs with floppy ears were more likely to develop these infections. The results from two swabs taken from the same ear were consistent for bacteria, but there was only moderate agreement for yeast. This suggests that ear cytology can be a reliable method for diagnosing ear infections in dogs.

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Abstract

Eighty-three dogs with clinical signs of otitis externa and with ear cytology revealing microbial organisms were studied. Samples were collected from both ears of each dog by inserting two swabs successively into each ear canal, rotating each once through 360 degrees and then rolling it out in a line on to a glass slide. For each animal, four single parallel smears (SPS) were made on one slide, which was then appropriately labelled to identify the animal as well as the order of the samples. The slides, one representing each animal, were subsequently stained with modified Wright's stain. Six high-power fields of every SPS were counted. Golden retrievers and West Highland white terriers were found to be predisposed to developing otitis externa (P=0.0006 and P=0.0123, respectively). Otitis externa occurred significantly more frequently in dogs with pendulous pinnae than in dogs with erect pinnae (P=0.0009). There was no significant difference between the first and the second samples with respect to the number of microorganisms found (P>0.1 for cocci and P>0.5 for rods and yeasts), and there was a substantial agreement between the results of the two successive swabs for the presence of cocci (kappa=0.765) and rods (kappa=0.705). For yeasts, the agreement was only moderate (kappa=0.581).

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Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/20605955/