Peer-reviewed veterinary case report
Retrospective application of transposon-directed insertion site sequencing to a library of signature-tagged mini-Tn5Km2 mutants of Escherichia coli O157:H7 screened in cattle.
- Journal:
- Journal of bacteriology
- Year:
- 2011
- Authors:
- Eckert, Sabine E et al.
- Affiliation:
- Roslin Institute and Royal (Dick) School of Veterinary Studies · United Kingdom
Abstract
Massively parallel sequencing of transposon-flanking regions assigned the genotype and fitness score to 91% of Escherichia coli O157:H7 mutants previously screened in cattle by signature-tagged mutagenesis (STM). The method obviates the limitations of STM and markedly extended the functional annotation of the prototype E. coli O157:H7 genome without further animal use.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/21278291/