Peer-reviewed veterinary case report
Reverse line blot hybridization with species-specific oligonucleotide probes: application to piroplasm detection.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2015
- Authors:
- Hurtado, Ana
- Affiliation:
- Department of Animal Health · Spain
Abstract
Reverse line blot (RLB) hybridization has become a well-established and widely used method for the multiplex identification of several Babesia and Theileria species in hosts and tick vectors. The procedure is based on the simultaneous PCR amplification of a polymorphic region of the 18S rRNA gene from different piroplasms followed by identification of the individual species by hybridization to species-specific oligonucleotide probes covalently linked to a nylon membrane in a macroarray format.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25399097/