Peer-reviewed veterinary case report
Reverse transcription loop-mediated isothermal amplification for rapid detection of the newly emerged poultry Flavivirus.
- Journal:
- Folia microbiologica
- Year:
- 2013
- Authors:
- Li, Zhaolong et al.
- Affiliation:
- Department of Animal Husbandry and Veterinary Medicine · China
Plain-English summary
A new virus called Poultry Flavivirus (PF) has recently emerged in China, causing serious illness and death in birds, particularly leading to a condition known as egg drop syndrome. Researchers developed a test called reverse transcription loop-mediated isothermal amplification (RT-LAMP) that can quickly and accurately detect this virus. This test is much more sensitive than traditional methods, able to find very small amounts of the virus without getting confused by other poultry viruses. It can be done without complicated equipment, making it useful for diagnosing the virus in real-world settings. Overall, this new test is an effective tool for identifying Poultry Flavivirus in birds.
Abstract
Poultry Flavivirus (PF) was a recently emerged virus with high morbidity rates and mortality rates in China. It is the causative agent of egg drop syndrome at present. Development of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was the most efficient way to prevent and control the PF disease. The assay was performed at 64 °C for 45 min, using six specific primers that recognized eight targets of the PF E gene. The RT-LAMP assay, compared to conventional reverse transcription polymerase chain reaction, has 100-fold-greater sensitivity, with a detection limit of 1 × 10(-3) copies per μL RNA and no cross-reaction with poultry other viruses. The RT-LAMP assay is a valuable tool for detected PF without requiring any sophisticated equipment, and the detection has potential usefulness for clinical diagnosis in the field.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/23152303/