Ribosomal protein L32 contributes to the growth, antibiotic resistance and virulence of.

Abstract

is the pathogen that causes Glässer's disease in pigs, which is characterized by fibrinous polyserositis, arthritis and meningitis. Research on ribosomal protein L32 in microorganisms has mainly focused on regulating gene transcription and translation, but its effect on bacterial virulence is unclear. The role ofgene inis not clear, and in order to study the function ofgene, a suicide plasmid-mediated natural transformation method was used to construct agene deletion mutant. We found that althoughwas shown to be non-essential for cell proliferation, the growth curve of Δis clearly different compared with that of ZJ1208. Δproduced more outer membrane vesicles (OMVs) with a variety of irregular shapes, but produced similar biofilm to the parental strain. Δis more sensitive to osmotic pressure, oxidation pressure and heat shock stress. Meanwhile, Δis significantly more susceptible to antimicrobials such as spectinomycin, apramycin, sulfafurazole, but not to other antibiotics used in this study. In the mouse challenge experiment, the mortality of mice infected with the mutant strain decreased by 40% compared to those infected with the wild-type strain, indicating thatis a virulence-associated factor which contributes to bacterial fitness in host environments. The above results show thatis important for the growth, stress resistance and virulence of, and this study also confirms for the first time thatplays an important role in antibiotic resistance against aminoglycosides and sulfonamides.