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Peer-reviewed veterinary case report

RpoB H481Y Rifampicin Resistance Mutation-Associated Oxidative Stress Sensitivity Reduces the Virulence of Staphylococcus aureus.

Journal:
Microbiology and immunology
Year:
2025
Authors:
Kano, Tomonori et al.
Affiliation:
Graduate School of Medicine · Japan

Abstract

In this study, we have established a Staphylococcus aureus RpoB H481Y mutant strain and demonstrated that it is sensitive to menadione or hydrogen peroxide-induced oxidative stress and exhibits reduced virulence against a silkworm infection model. Furthermore, the reduced virulence of the RpoB H481Y mutant was abrogated in the presence of N-acetylcysteine, a reactive oxygen species scavenger. These results suggest that oxidative stress sensitivity caused by the RpoB H481Y rifampicin resistance mutation attenuates the virulence of S. aureus.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/39616518/