Salvaging the zone of stasis by simvastatin: an experimental study in rats.

Abstract

Salvaging the zone of stasis is important for burn researchers because this can prevent an increase in the depth and width of the injured area. Statin analogues have many pleiotropic effects on the vessel walls and the coagulation and fibrinolytic systems. In this study, we investigated the effects of simvastatin, a statin analogue, administered to rats burned with a metal comb. No treatment was given to the control group (n = 10). Simvastatin was given at a dose of 5 mg/kg/d by intraperitoneal injection in treatment group (n = 10) for 7 days. Phosphate-buffered saline was given 1 mg/kg/d by intraperitoneal injection in sham group (n = 10). The groups were randomly divided into two subgroups (n = 5) for evaluation at 24 hours and 7 days. It was observed that there were necrotic areas and viable interspaces in both the experimental and control groups at 24 hours. The interspaces progressed to necrotic areas in the control and sham groups at 7 days. However, viable interspaces were separated from necrotic areas clearly in the treatment group at 7 days. In the samples taken from interspaces at 24 hours, positive staining for thrombomodulin (TM) for all groups was noted. In the samples taken from the control and phosphate-buffered saline groups at 7 days, there was negative staining for TM. However, in the samples taken from interspaces of the treatment group, positive staining for TM was observed. The conclusion of this study was that simvastatin potently increased endothelial TM expression in the zone of stasis and preserved the zone.