Peer-reviewed veterinary case report
New dot ELISA test detects canine distemper virus in dogs
By Li, Zhi et al.·Published in Canadian journal of veterinary research = Revue canadienne de recherche veterinaire·2013·Dalian Municipal Central Hospital, China·View original on PubMed →
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Original publication title: Sandwich-dot enzyme-linked immunosorbent assay for the detection of canine distemper virus.
- Species:
- dog
Plain-English summary
A new test called dot ELISA was developed to quickly detect canine distemper virus (CDV) in dogs showing symptoms of the disease. In a study of 56 dogs suspected to have CD, the dot ELISA was able to identify the virus in blood and eye samples with high accuracy, detecting CDV in 91% of blood samples and 88% of eye samples. This test is not only reliable but also faster than traditional methods, making it useful for diagnosing sick dogs and monitoring the disease in populations.
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Abstract
A sandwich-dot enzyme-linked immunosorbent assay (dot ELISA) was developed for the detection of canine distemper virus (CDV). In 56 dogs suspected to have CD the rates of detection of CDV antigen in samples of blood lymphocytes and palpebral conjunctiva by dot ELISA and ELISA were, respectively, 91% (49/54) and 81% (44/54) for the lymphocyte samples and 88% (28/32) and 75% (24/32) for the conjunctival samples. The CDV detection limits were 10 ng/50 μL for dot ELISA and 40 ng/50 μL for ELISA. The reliability of dot ELISA relative to electron microscopy was 96% with 22 samples: all 21 samples in which CDV particles were observed by electron microscopy yielded positive results with dot ELISA; the single sample in which particles were not observed yielded false-positive results with dot ELISA. The results indicate that the dot ELISA developed can serve as a reliable rapid diagnostic test in suspected cases of CD and also be useful for epidemiologic surveillance of the disease.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/24124274/