Secondary Metabolites from the Nematode-Trapping Fungus <i>Dactylellina haptotyla</i> YMF1.03409.

Abstract

As a representative nematode-trapping fungus, <i>Dactylellina haptotyla</i> can capture and kill nematodes by producing traps, known as adhesive knobs. In this paper, the strain of <i>D. haptotyla</i> YMF1.03409 was studied by means of medium screening, fermentation, and purification and identification of crude extracts. Eighteen compounds were obtained from <i>D. haptotyla</i> YMF1.03409, including two new metabolites, nosporins C (<b>1</b>) and D (<b>2</b>). The known metabolites were identified to be 3-chloro-4-methoxybenzaldehyde (<b>3</b>), 3-chloro-4-methoxybenzoic acid (<b>4</b>), 2-chloro-1-methoxy-4-(methoxymethyl)benzene (<b>5</b>), 3-hydroxy-3-methyloxindole (<b>6</b>), nicotinic acid (<b>7</b>), succinic acid (<b>8</b>), 3,4-dihydroxybutanoic acid (<b>9</b>), 5'-O-methyladenosine (<b>10</b>), uridine (<b>11</b>), 2'-deoxyuridine (<b>12</b>), thymidine (<b>13</b>), 3-(phenylmethyl)-2,5-morpholinedione (<b>14</b>), methyl-β-D-glucopyranoside (<b>15</b>), 1,2-benzenedicarboxylic acid bis(2-methyl heptyl) ester (<b>16</b>), β-sitosterol (<b>17</b>), and 3β,6α-diol-stigmastane (<b>18</b>). The bioactive assay showed that these compounds had no obvious nematicidal activity against the nematodes <i>Meloidogyne incognita</i> and <i>Panagrellus redivivus</i>.