Peer-reviewed veterinary case report
Segmentation expression of capsid protein as an antigen for the detection of avian nephritis virus infection in chicken flocks.
- Journal:
- Journal of virological methods
- Year:
- 2012
- Authors:
- Zhao, W et al.
- Affiliation:
- School of Agriculture and Biology · China
- Species:
- bird
Plain-English summary
Researchers have developed a new test to help detect an infection caused by the avian nephritis virus (ANV) in chickens, which can lead to kidney problems and slow growth. This test looks for specific antibodies in the blood of chickens, which can indicate whether they have been infected with the virus. In a study involving 960 chickens aged 10 to 25 days from China, nearly half of the samples tested positive for ANV. The new test, called P1-ELISA, shows promise for diagnosing ANV infections and could be useful for monitoring the health of chicken flocks. Overall, the test appears to work well for identifying infected chickens.
Abstract
Subclinical pathological changes in the kidneys of broiler chickens and suppression of growth caused by the avian nephritis virus (ANV) affect poultry flocks worldwide. A test for detection of virus-specific antibodies in serum would be useful for epidemiological investigations, however the poor propagation in cell cultures has restricted the development of serological tests based on the use of ANV particles as antigens. An enzyme-linked immunosorbent assay (ELISA) was developed for detection of ANV-specific antibodies in chicken serum, using a recombinant protein antigen prepared by segmentation expression of the capsid protein antigen epitope of ANV (HM029238) transfected into Escherichia coli. The expressed fusion protein was detected by Western blotting with ANV-positive serum, and the optimal immunoreactive fusion P1 protein was determined. Using the optimized P1-ELISA, ANV-specific antibodies were detected in commercial chicken flocks aged 10-25 days obtained from the Liaoning Province, China. Out of 960 serum samples, 459 (47.8%) were positive for infection with ANV. These results indicate that the P1-ELISA is helpful for preliminary serological diagnosis of ANV infection, and could be used to for screening in ANV infection and for determining antibodies against ANV.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/22001271/