Peer-reviewed veterinary case report
Sensitive detection of porcine circovirus 3 by droplet digital PCR.
- Journal:
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
- Year:
- 2019
- Authors:
- Liu, Yuqi et al.
- Affiliation:
- Research Institute of Food Safety and Nutrition · China
Abstract
Porcine circovirus 3 (PCV-3) is a newly emerging virus that poses a potential threat to the swine industry. We developed a sensitive assay utilizing droplet digital PCR (ddPCR) to detect PCV-3. Specificity of the assay was confirmed by the failure of amplification of DNA of other relevant viruses. The detection limit for ddPCR was 1 copy/μL, 10 times greater sensitivity than TaqMan real-time PCR (rtPCR). Both methods showed a high degree of linearity, although TaqMan rtPCR showed less sensitivity than ddPCR for clinical detection. Our findings indicate that ddPCR might offer faster and improved analytical sensitivity for PCV-3 detection.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/31046639/