Peer-reviewed veterinary case report
Finding new viruses in dog livers with VIDISCA-454 technique
By van der Heijden, Mitzi et al.·Published in Journal of virological methods·2012·Department of Clinical Sciences of Companion Animals, Netherlands·View original on PubMed →
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Original publication title: Sequence-independent VIDISCA-454 technique to discover new viruses in canine livers.
- Species:
- dog
Plain-English summary
A dog with acute hepatitis was studied to find out if a virus was causing the illness. Researchers used a special technique to analyze liver tissue and discovered that Canine adenovirus type 1 (CAV-1) was the culprit. This finding is important because it could lead to the development of specific treatments and vaccines for dogs suffering from idiopathic hepatitis, a condition where the cause is unknown. The study shows that this new method can effectively identify viruses even without prior knowledge of their genetic makeup.
People also search for: dog hepatitis symptoms · canine adenovirus treatment · what causes liver disease in dogs
Abstract
In many mammals, viruses cause hepatitis. Despite many efforts a specific virus responsible for canine idiopathic hepatitis has not been identified. The discovery of a viral etiology in canine hepatitis will promote the development of specific drugs and vaccines for the treatment of idiopathic hepatitis in dogs. The objective of this study was the application of the sequence-independent Virus Discovery cDNA-amplified fragment length polymorphism (VIDISCA) technique combined with high through-put sequencing on a Roche-454 sequencer to identify unknown viruses. Liver tissue of a dog with idiopathic acute hepatitis was cultured on a canine liver cell line and the cell culture medium was submitted to the VIDISCA-454 technique. Without prior knowledge of the viral species involved, this technique identified Canine adenovirus type 1 (CAV-1) as the infecting agent. This demonstrates the power of VIDISCA-454 to identify viruses, independent of preliminary information about the genomic sequence. Consequently, the strategy of propagation in this cell line followed by the VIDISCA-454 technique is valuable to identify the viral etiology of idiopathic hepatitis in dogs.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/22664180/