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Peer-reviewed veterinary case report

Sequencing of avian influenza virus genomes following random amplification.

Journal:
BioTechniques
Year:
2007
Authors:
Afonso, Claudio L
Affiliation:
U.S. Department of Agriculture · United States
Species:
bird

Abstract

Increasing surveillance for the avian influenza virus (AIV) has underscored the need for quickly and precisely characterizing isolates of this highly variable target. Random amplification, sequencing, and assembly of total RNA from nonpurified virus overcomes the need for specific primers for DNA microarray or PCR protocols.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/17824386/