Peer-reviewed veterinary case report
Serodiagnosis of grass carp reovirus infection in grass carp Ctenopharyngodon idella by a novel Western blot technique.
- Journal:
- Journal of virological methods
- Year:
- 2013
- Authors:
- He, Yongxing et al.
- Affiliation:
- Shanghai Ocean University · China
Plain-English summary
Grass carp, a type of fish, can suffer from a serious illness called grass carp hemorrhagic disease, which is caused by an infection from grass carp reovirus (GCRV). Researchers have been trying to find better ways to diagnose this infection, as existing methods have limitations. In this study, scientists created a new test using a technique called Western blot, which can specifically detect antibodies related to the virus in the fish's blood. They compared this new method to a traditional test and found that it worked well on samples from sick fish collected during an outbreak in 2011. Overall, the new Western blot technique could be a reliable way to diagnose GCRV infection in grass carp.
Abstract
Frequent outbreaks of grass carp hemorrhagic disease, caused by grass carp reovirus (GCRV) infection, pose as serious threats to the production of grass carp Ctenopharyngodon idella. Although various nucleic acids-based diagnostic methods have been shown effective, lack of commercial monoclonal antibody against grass carp IgM has impeded the development of any reliable immunoassays in detection of GCRV infection. The present study describes the preparation and screening of monoclonal antibodies against the constant region of grass carp IgM protein, and the development of a Western blot (WB) protocol for the specific detection of antibodies against outer capsid VP7 protein of GCRV that serves as antibody-capture antigen in the immunoassay. In comparison to a conventional RT-PCR method, validity of the WB is further demonstrated by testing on clinical fish serum samples collected from a grass carp farm in Jiangxi Province during disease pandemic in 2011. In conclusion, the WB technique established in this study could be employed for specific serodiagnosis of GCRV infection.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/23942340/