Peer-reviewed veterinary case report
SEROLOGIC STUDIES OF AFRICAN HORSE-SICKNESS VIRUS WITH EMPHASIS ON NEUTRALIZATION TEST IN TISSUE CULTURE.
- Journal:
- Canadian journal of comparative medicine and veterinary science
- Year:
- 1965
- Authors:
- HAZRATI, A & OZAWA, Y
- Species:
- rodent
Plain-English summary
This study looked at a virus that affects horses, called African horse-sickness virus, using special cell cultures instead of mice for testing. Researchers found that the best way to neutralize the virus was to mix it with a serum solution and let it sit at a warm temperature for an hour. They discovered that the antibodies created in rabbits and guinea pigs were just as effective as those made using a different method involving mouse brain. The researchers also identified different strains of the virus from Asia, finding that they were closely related and similar to a specific type of the virus. Overall, the study showed that using these cell cultures can effectively help in understanding and identifying this virus.
Abstract
In place of mice, monkey kidney stable (MS) cell cultures were used successfully in serologic studies of African horse-sickness virus.The maintenance medium containing 2% serum was chosen as the virus diluent. Maximum neutralization occurred after 1-hour incubation at 37 C., and maintained the same titer during an additional 4-hour incubation period. No significant difference was observed between neutralization titers titrated using the same antiserum mixed with two different passage levels of virus. Rabbit and guinea pig antiserums prepared using virus grown in MS cell cultures had antibody titer as high as those prepared in the same manner using mouse brain suspension. African horse-sickness virus strains isolated in Asia were serologically identified using a standard neutralization technique in tissue culture. All the strains were closely related to each other and all had antigenic similarity to Type 6 virus (strain 114).
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/14300857/