Peer-reviewed veterinary case report
Serological diagnosis of feline coronavirus infection by immunochromatographic test.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2015
- Authors:
- Takano, Tomomi & Hohdatsu, Tsutomu
- Affiliation:
- School of Veterinary Medicine · Japan
- Species:
- cat
Abstract
The immunochromatographic assay (ICA) is a simple antibody-antigen detection method, the results of which can be rapidly obtained at a low cost. We designed an ICA to detect anti-feline coronavirus (FCoV) antibodies. A colloidal gold-labeled recombinant FCoV nucleocapsid protein (rNP) is used as a conjugate. The Protein A and affinity-purified cat anti-FCoV IgG are blotted on the test line and the control line, respectively, of the nitrocellulose membrane. The specific detection of anti-FCoV antibodies was possible in all heparin-anticoagulated plasma, serum, whole blood, and ascitic fluid samples from anti-FCoV antibody positive cats, and nonspecific reaction was not noted in samples from anti-FCoV antibody negative cats.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25720468/