Peer-reviewed veterinary case report
Antibodies to lens proteins found in American Cocker Spaniels
By Kanemaki, Nobuyuki et al.·Published in Veterinary ophthalmology·2015·Veterinary Teaching Hospital, Japan·View original on PubMed →
PetCaseFinder translated the abstract of this peer-reviewed paper into plain English so pet owners can read it. We do not publish original research — every detail traces back to the citation above. How we work →
Original publication title: Serum antibodies against βH-crystallins in the American Cocker Spaniel.
- Species:
- dog
Plain-English summary
A group of 73 American Cocker Spaniels was tested for antibodies related to a specific protein in the eye called βA1-crystallin, which may be linked to cataracts. Some dogs had cataracts, while others had normal lenses or uveitis (inflammation of the eye). The study found that about two-thirds of the dogs had antibodies against certain proteins, but the presence of these antibodies did not directly relate to the severity of cataracts. However, specific antibodies were only found in dogs with cataracts, suggesting a possible connection.
People also search for: American Cocker Spaniel cataracts treatment · dog eye problems antibodies · what causes cataracts in dogs
Abstract
OBJECTIVE: To detect antibodies for lens βH-crystallins in the serum from the American Cocker Spaniel (ACS) presenting with and without cataracts and with and without uveitis. ANIMAL STUDIED: Seventy-three American Cocker Spaniels and six normal Beagles. PROCEDURES: Sera were collected from 73 ACSs, including those with normal lenses and those with cataracts, or uveitis. Fractionated, normal Beagle lens βH-crystallins were separated by one- or two-dimensional electrophoresis. The separated lens βH-crystallins were used on immunoblots as sentinel substrates against which the ACS sera were tested for the presence of antibodies against βH-crystallins. RESULTS: Sera from approximately two-thirds of study animals contained antibodies to some βH-crystallin polypeptides, but reactivity varied among patients. Contrary to some hypotheses, serum antibodies to groups of βH-crystallins did not relate to the stages of cataract. However, detailed analysis by two-dimensional immunoblotting and mass spectrometry showed that three spots originating from βA1-crystallin were detected only in sera from cataract patients. CONCLUSION: Serum antibodies to βA1-crystallin may be associated with the development of cataract.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/24171837/