Peer-reviewed veterinary case report
Blood tests for IL6 and SLPI can predict sepsis in dogs with pyometra
By Sasidharan, Jisna Kuttiyeri et al.·Published in Reproduction in domestic animals = Zuchthygiene·2023·Indian Council of Agricultural Research (ICAR)-Indian Veterinary Research Institute (IVRI), India·View original on PubMed →
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Original publication title: Serum concentrations of secretory leukocyte protease inhibitor and IL6 can predict the onset of sepsis in pyometra bitches.
- Species:
- dog
Plain-English summary
A group of female dogs with a serious uterine infection called pyometra were studied to see if certain blood markers could help predict if they were developing sepsis, a life-threatening infection. Researchers found that levels of two proteins, IL6 and SLPI, were significantly higher in dogs with sepsis compared to those without it. This means that measuring these proteins could help veterinarians identify which dogs are at greater risk for sepsis and tailor their treatment accordingly. By using these markers alongside other tests, vets can better manage the health of dogs suffering from pyometra.
People also search for: dog pyometra symptoms · sepsis in dogs treatment · IL6 levels in dogs
Abstract
As onset of sepsis adversely affects the prognosis of canine pyometra, finding biomarkers that would distinguish sepsis status would be useful in the clinical management. Accordingly, we hypothesized that differential expression of endometrial transcripts and circulating concentration of certain inflammatory mediators would discriminate pyometra-led sepsis (P-sepsis+) from those of pyometra without sepsis (P-sepsis-). Bitches with pyometra (n = 52) were classified into P-sepsis+ (n = 28) and P-sepsis- (n = 24) based on vital clinical score and total leukocyte count. A group of non-pyometra bitches (n = 12) served as control. The relative fold changes in the transcripts of IL6, IL8, TNFα, IL10, PTGS2, mPGES1 and PGFS, SLPI, S100A8, S100A12 and eNOS were determined by quantitative polymerase chain reaction. Furthermore, the serum concentrations of IL6, IL8, IL10, SLPI and prostaglandin Fmetabolite (PGFM) were assayed by ELISA. The relative fold changes in S100A12 and SLPI and mean concentrations of IL6 and SLPI were significantly (p < .05) higher in P-sepsis+ than that of P-sepsis- group. Receiver operating characteristic analysis revealed that serum IL6 had a diagnostic sensitivity of 78.6% and a positive likelihood ratio (LR+) of 2.09, at a cut-off value of 15.7 pg/mL to diagnose P-sepsis+ cases. Similarly, serum SLPI had a sensitivity of 84.6% and an LR+ of 2.23, at a cut-off value of 2.0 pg/mL. It was concluded that SLPI and IL6 would serve as putative biomarkers for pyometra-led sepsis in bitches. Monitoring SLPI and IL6 would be a useful adjunct to the established haemato-biochemical parameters in customizing the treatment strategies and arriving at the decision for management of pyometra bitches with critical illness.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/37010813/