Serum malondialdehyde level and activity of total antioxidant status of dogs with age-related cataract.

Abstract

STUDY OBJECTIVE: determination of malondialdehyde (MDA) level and total antioxidant status (TAS) in the serum of dogs with age-related cataract. MATERIAL AND METHODS: Forty dogs, 10-16 years of age, which were diagnosed with age-related cataract were examined. The control group consisted of 12 dogs, 8-13 years of age, without cataract. MDA was determined using a Perkin-Elmer LS 30 luminescence spectrometer using the method with thiobarbituric acid (TBA). TAS was determined using the colorimetric method with a set of Randox Total Antioxidant Status reagents. RESULTS: The results show a higher level of MDA with statistically significant difference and lower activity of TAS in the serum of dogs with age-related cataract. CONCLUSIONS: The results obtained indicate higher systemic lipid peroxidation and weakening of the general defense system in dogs with age-related cataract. These results suggest that such conditions may have an effect on the development of age-related cataract and are consistent with the theory of free radical age-related cataract development.