Peer-reviewed veterinary case report
Shaved margin histopathology and imprint cytology for assessment of excision in canine mast cell tumors and soft tissue sarcomas.
- Journal:
- Veterinary surgery : VS
- Year:
- 2017
- Authors:
- Milovancev, Milan et al.
- Affiliation:
- Department of Clinical Sciences
- Species:
- dog
Plain-English summary
This study looked at different ways to check if all of a tumor was removed during surgery in dogs with certain types of skin tumors, specifically mast cell tumors and soft tissue sarcomas. Researchers tested three methods: imprint cytology, which involves pressing a slide to the surgical edge to collect cells; shaved margin histopathology, where a small area of tissue is taken from the wound before closing it; and the standard radial section histopathology, which is the usual method for examining tissue samples. They found that the methods often gave different results, especially for mast cell tumors, meaning that one method might suggest tumor cells were left behind while another might not. Overall, while the new methods can be used, they don't always agree with the standard method, and more research is needed to see how these differences might affect the chances of the tumor coming back.
Abstract
OBJECTIVE: To determine the feasibility and agreement of margin assessment by imprint cytology, shaved margin histopathology, and radial section histopathology in canine cutaneous and subcutaneous mast cell tumors (MCT) and soft tissue sarcomas (STS). STUDY DESIGN: Prospective clinical study. SAMPLE POPULATION: Three hundred and forty margins from 72 excised tumors (52 MCT and 20 STS) in 54 client-owned dogs. METHODS: Imprint cytology samples were acquired by pressing glass slides to the cut surgical margin of the freshly excised surgical specimen. Shaved margin samples were obtained from the patient wound bed using a scalpel immediately prior to closure. Radial section histopathology was performed as part of routine histopathologic processing. All margins were assessed as either positive or negative for presence of tumor cells at the surgical margin. Agreement among methods was calculated using Fleiss Kappa coefficients and an association of method, margin direction, and tumor type with positive margin status was evaluated using a general linear mixed model. RESULTS: Positive margin detection rates differed for MCT (imprint cytology 21%, radial section histopathology 9%, and shaved margin histopathology 3%; P < .0001) but not for STS. Intermethod agreement was poor (Fleiss Kappa = 0.051 and 0.176 for MCT and STS, respectively). Margin direction did not influence margin status for either tumor type. CONCLUSION: Imprint cytology and shaved margin histopathology are feasible, but their results are frequently disparate from routine radial section histopathology. Future studies are needed to evaluate the correlation of each method with local recurrence rates.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/28460419/