Peer-reviewed veterinary case report
Rapid test to detect severe fever with thrombocytopenia syndrome
By Keita Ishijima et al.·Published in Journal of Veterinary Medical Science·2023·View original on Semantic Scholar →
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Original publication title: Simple and rapid detection of severe fever with thrombocytopenia syndrome virus in cats by reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay using a dried reagent
- Species:
- cat
Plain-English summary
A group of cats was tested for a serious virus called severe fever with thrombocytopenia syndrome virus (SFTSV), which can cause severe illness and even death. Researchers developed a quick test that doesn't require complicated steps to detect the virus in cats, using both liquid and dried reagents. The test successfully identified the virus in nearly all infected cats, even a few days after they were exposed. This new method is easier to use in veterinary clinics, making it faster to diagnose and help prevent the spread of the virus to humans.
People also search for: cat fever symptoms · SFTSV in cats · quick test for cat viruses · how to treat cat infections · severe fever with thrombocytopenia syndrome in pets
Abstract
Severe fever with thrombocytopenia syndrome virus (SFTSV) causes lethal hemorrhagic diseases in human, cats, and dogs. Several human cases involving direct transmission of SFTSV from diseased animals have been reported. Therefore, rapid diagnosis in veterinary clinics is important for preventing animal-to-human transmission. Previously, we developed a simplified reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for human that does not require RNA extraction for detecting the SFTSV genome. In this study, we improved the simplified RT-LAMP assay for cats by introducing a dried reaction reagent and investigated the applicability of this method for diagnosing SFTS in cats. SFTSV RNA was detected in 11 of 12 cats naturally infected with SFTSV by RT-LAMP assay using both liquid and dried reagents. The RT-LAMP assay using liquid and dried reagents was also applicable to the detection of SFTSV genes 3–4 days after challenge in cats experimentally infected with SFTSV. The minimum copy number of SFTSV genes for 100% detection using the RT-LAMP assay with liquid and dried reagents was 4.3 × 104 and 9.6 × 104 copies/mL, respectively. Although the RT-LAMP assay using the dried reagent was less sensitive than that using the liquid reagent, it was sufficiently sensitive to detect SFTSV genes in cats with acute-phase SFTS. As the simplified RT-LAMP assay using a dried reagent enables detection of SFTSV genes more readily than the assay using a liquid reagent, it is applicable for use in veterinary clinics.
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Search related cases →Original publication on Semantic Scholar: https://www.semanticscholar.org/paper/36653150