Peer-reviewed veterinary case report
Simple differentiation of avirulent and virulent strains of avian paramyxovirus serotype-1 (Newcastle disease virus) by PCR and restriction endonuclease analysis in Japan.
- Journal:
- The Journal of veterinary medical science
- Year:
- 2012
- Authors:
- Mase, Masaji & Kanehira, Katsushi
- Affiliation:
- National Institute of Animal Health · Japan
- Species:
- bird
Abstract
To differentiate the avirulent from virulent strains of avian paramyxovirus serotype-1 (APMV-1, Newcastle disease virus), PCR and restriction endonuclease analysis (REA) was employed. Primer sequences were used to amplify a 766-base pair fragment that included the fusion protein cleavage site. REA of PCR products generated by Hin1 I and Apa I enabled the differentiation of avirulent field and vaccine strains from more virulent field strains of APMV-1 in Japan.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/22814085/