Peer-reviewed veterinary case report
Simultaneous detection of antibodies to five simian viruses in nonhuman primates using recombinant viral protein based multiplex microbead immunoassays.
- Journal:
- Journal of virological methods
- Year:
- 2011
- Authors:
- Liao, Qi et al.
- Affiliation:
- NanKai University · China
Plain-English summary
Researchers have developed a new testing method to check for infections in nonhuman primates, which are often kept in controlled environments to prevent disease. This new method uses specially made proteins from viruses instead of live viruses, making it safer and easier to use. It can detect antibodies to five different viruses at the same time, which helps to quickly determine if the animals are infected. The results showed that this new test works well and is just as reliable as older methods. Overall, this new approach is an effective way to monitor the health of these primates.
Abstract
Routine screening for infectious agents is critical in establishing and maintaining specific pathogen free (SPF) nonhuman primate (NHP) colonies. More efficient, higher throughput, less costly reagent, and reduced sample consumption multiplex microbead immunoassays (MMIAs) using purified viral lysates have been developed previously to address some disadvantages of the traditional individual enzyme-linked immunosorbent assay (ELISA) methods. To overcome some of the technical and biosafety difficulties in preparing antigens from live viruses for viral lysate protein based MMIAs, novel MMIAs using recombinant glycoprotein D precursor (gD) protein of herpesvirus B and four viral gag proteins of simian immunodeficiency virus (SIV), simian T Cell lymphotropic virus (STLV), simian foamy virus (SFV), and simian betaretrovirus (SRV) as antigens have been developed in the current study. The data showed that the recombinant viral protein based MMIAs detected simultaneously antibodies to each of these five viruses with high sensitivity and specificity, and correlated well with viral lysate based MMIAs. Therefore, recombinant viral protein based MMIA is an effective and efficient routine screening method to determine the infection status of nonhuman primates.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/21945221/