Peer-reviewed veterinary case report
Test to detect multiple viruses causing dog respiratory disease
By Jeoung, Hye-Young et al.·Published in The Journal of veterinary medical science·2013·Animal, South Korea·View original on PubMed →
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Original publication title: Simultaneous detection of canine respiratory disease associated viruses by a multiplex reverse transcription-polymerase chain reaction assay.
- Species:
- dog
Plain-English summary
A group of 50 dogs showing symptoms of respiratory illness, such as coughing and difficulty breathing, were tested for three viruses that can cause these issues: canine distemper virus, canine respiratory coronavirus, and canine influenza virus. Researchers developed a new test that can detect all three viruses at once using nasal swabs. This test was found to be just as accurate as existing methods, making it a reliable option for diagnosing these viral infections in dogs. With this new testing method, veterinarians can quickly identify the cause of respiratory problems and provide appropriate treatment.
People also search for: dog coughing treatment · canine influenza virus symptoms · how to test for dog respiratory disease
Abstract
A multiplex reverse transcription polymerase chain reaction (mRT-PCR) assay was developed for the simultaneous detection of canine distemper virus (CDV), canine respiratory coronavirus (CRCoV) and canine influenza virus (CIV). These viral pathogens are all causative agents of canine infectious respiratory disease (CIRD). The sensitivity and specificity of the mRT-PCR were determined by comparing it to a rapid antigen test (RAT) or immuno-chromatography test kit and reverse transcription-polymerase chain reaction (RT-PCR) in the detection of CDV, CRCoV and CIV antigens present in 100 clinical samples (nasal swabs and whole blood samples) from 50 dogs with respiratory disease symptoms. This study revealed that mRT-PCR had almost exactly the same performance or results were almost 100% in agreement with that of RT-PCR and RAT both in terms of the assay sensitivity and specificity which was more highly evident in detecting CIV, CDV and CRCoV antigens present in canine nasal swab samples. Therefore, this assay could be a better alternative for the definitive and simultaneous ante-mortem detection of the three viral pathogens that cause CIRD by using nasal swabs.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/22971595/