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Peer-reviewed veterinary case report

Simultaneous determination of lincomycin, spiramycin, tylosin and tiamulin in animal feed using liquid chromatography coupled to mass spectrometry after a microbial assay

Journal:
Journal of Veterinary Research
Year:
2025
Authors:
Przeniosło-Siwczyńska Monika et al.
Affiliation:
1Department of Microbiological Research of Food and Feed, Department of Chemical Research of Food and Feed, National Veterinary Research Institute, 24-100Puławy, Poland · PL

Abstract

To monitor the occurrence of antibiotics in feed, a two-step control strategy is often adopted of screening by microbiological inhibition followed by confirmation by chromatographic techniques. This study is devoted to the development of a reliable method for simultaneous determination of lincomycin, spiramycin, tylosin and tiamulin in animal feed using liquid chromatography coupled to mass spectrometry after a microbial assay.

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Original publication: https://doi.org/10.2478/jvetres-2025-0073