siRNA targetinggene encoding ATPase α, the ligand of Peg fimbriae, reducedEnteritidis adhesion.

Abstract

enterica serovar Enteritidis (. Enteritidis) is a zoonotic pathogen that can infect both humans and animals. Among the 13 types of fimbrial operons in. Enteritidis, the highly conserved Peg fimbriae play a crucial role in the adhesion and invasion of. Enteritidis into host cells but are not well studied. In this study, we identified the ATP synthase subunit alpha (ATPase &#x3b1;) as a ligand of Peg fimbriae using ligand blotting and mass spectrometry techniques. We confirmed thebinding of ATPase &#x3b1; to the purified adhesion protein (PegD). Furthermore, we used siRNA to suppress the expression of ATPase &#x3b1; genein Leghorn male hepatoma (LMH) cells, which resulted in a significant reduction in the adhesion rate of. Enteritidis to the cells (&#x2009;<&#x2009;0.05). The findings in this study provide insight into the mechanism of. Enteritidis infection through Peg fimbriae and highlight the importance of ATPase &#x3b1; in the adhesion process.Ligand blotting was performed to screen the ligand ofEnteritidis Peg fimbriae.Binding assay confirmed that ATPase &#x3b1; is the ligand of the Peg fimbriae.siRNA targeting ATPase &#x3b1; gene () significantly reduced. Enteritidis adhesion.