Peer-reviewed veterinary case report
Histamine release in dog skin with atopic dermatitis after allergen
By Hammerberg, B et al.·Published in Veterinary dermatology·2001·Department of Microbiology, United States·View original on PubMed →
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Original publication title: Skin mast cell histamine release following stem cell factor and high-affinity immunoglobulin E receptor cross-linking in dogs with atopic dermatitis.
- Species:
- dog
Plain-English summary
A group of dogs with atopic dermatitis (a skin allergy condition) showed increased skin reactions when tested with a substance called stem cell factor (SCF) and an antibody that targets IgE, which is involved in allergic responses. The atopic dogs had larger skin reactions compared to normal dogs, indicating their mast cells (a type of immune cell) were more reactive. This suggests that the presence of SCF in their skin might make their mast cells more likely to release histamine, which causes itching and inflammation. Understanding this could help improve treatments for dogs suffering from skin allergies.
People also search for: dog skin allergies treatment · why is my dog itching · atopic dermatitis in dogs · mast cell activation in dogs
Abstract
Stem cell factor (SCF) influences mast cell activation and inflammatory mediator release, and is elevated in tissues undergoing allergic inflammation. Wheal formation in response to the injection of SCF or anti-immunoglobulin (Ig)E antibody injection was compared between normal (n = 10) and nonlesional atopic (n = 10) canine skin. In situ SCF secretion was compared between lesional and nonlesional skin using immunohistochemistry. Histamine release by skin cell suspensions after stimulation with SCF, concanavalin A (ConA) or rabbit anticanine IgE antibodies was compared between normal and atopic dogs. All dogs exhibited strong responses to intradermal SCF injection at 10 and 50 ng mL(-1). Atopic dogs had significantly (P = 0.002) larger wheal responses to anti-IgE than normal dogs; but there was no difference in numbers of skin mast cells bearing IgE as detected by immunohistochemistry. Only atopic dogs exhibited interstitial deposition of SCF in both lesional and nonlesional skin specimens. Median histamine release stimulated by SCF in the absence of IgE from lesional skin cells was higher in atopic than normal dogs (P = 0.04). These experiments suggest that dermal SCF secretion could potentiate histamine release following IgE receptor cross-linking and thus, could be one of the explanations for the inherent mast cell hyperexcitability observed in canine atopic dermatitis.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/11844224/