Small-diameter bacterial cellulose-based vascular grafts for coronary artery bypass grafting in a pig model.

Abstract

Surgical revascularization is the gold standard in most cases of complex coronary artery disease. For coronary artery bypass grafting, autologous grafts are state-of-the-art due to their long-term patency. A non-negligible amount of patients lack suitable bypass material as a result of concomitant diseases or previous interventions. As a promising alternative, tissue-engineered vascular grafts made of biomaterials such as bacterial cellulose (BC) are gaining more and more attention. However, the production of small-diameter grafts (inner diameter < 6 mm) of application-oriented length (> 5 cm) and their <i>in vivo</i> long-term patency remain challenging. In this study, grafts of 20 cm in length with an inner diameter of 3 mm were generated in a custom-made bioreactor. To potentially improve graft compliance and, therefore <i>in vivo</i> patency, BC was combined with an embedded cobalt-chromium mesh. The grafts were designed for <i>in vivo</i> endothelialization and specific surgical properties and implanted as an aortocoronary bypass in a left anterior descending occluded pig model (<i>n</i> = 8). Coronary angiography showed complete patency postoperatively at 4 weeks. Following 4 weeks <i>in vivo</i>, the grafts were explanted revealing a three-layered wall structure. Grafts were colonized by smooth muscle cells and a luminal layer of endothelial cells with early formation of <i>vasa privata</i> indicating functional remodeling. These encouraging findings in a large animal model reveal the great potential of small-diameter BC grafts for coronary and peripheral bypass grafting.