Peer-reviewed veterinary case report
Smartphone-based multiplex 30-minute nucleic acid test of live virus from nasal swab extract.
- Journal:
- Lab on a chip
- Year:
- 2020
- Authors:
- Sun, Fu et al.
- Affiliation:
- Department of Electrical and Computer Engineering · United States
- Species:
- horse
Abstract
Rapid, sensitive and specific detection and reporting of infectious pathogens is important for patient management and epidemic surveillance. We demonstrated a point-of-care system integrated with a smartphone for detecting live virus from nasal swab media, using a panel of equine respiratory infectious diseases as a model system for corresponding human diseases such as COVID-19. Specific nucleic acid sequences of five pathogens were amplified by loop-mediated isothermal amplification on a microfluidic chip and detected at the end of reactions by the smartphone. Pathogen-spiked horse nasal swab samples were correctly diagnosed using our system, with a limit of detection comparable to that of the traditional lab-based test, polymerase chain reaction, with results achieved in ∼30 minutes.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/32334422/