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Peer-reviewed veterinary case report

Sonication induced intermediate in prion protein conversion.

Journal:
Protein and peptide letters
Year:
2008
Authors:
Zukas, Audrius A et al.
Affiliation:
United States Department of Agriculture · United States
Species:
rodent

Abstract

We have observed that hamster prion protein (PrP(C)) undergoes conformational changes on exposure to heat or sonication. If a sonication induced new conformer is seeded with a small amount of its abnormal pathogenic isoform (PrP(Sc)) it undergoes a significant conversion to a proteinase-resistant isoform. This suggests the presence of a third stable PrP conformer, which may be intermediate in the conversion of PrP(C) to PrP(Sc).

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Original publication: https://pubmed.ncbi.nlm.nih.gov/18289112/