sp. nov., isolated from equine clinical specimens.

Abstract

In this study, five strains of anaerobic, Gram-negative, rod-shaped bacteria were characterized phenotypically, biochemically and genotypically. These strains were clinically isolated from horse specimens in Japan. Phylogenetic trees constructed based on 16S rRNA gene sequence comparisons showed distinct clustering of the five strains with the type strains of closely related species. The genomic DNA G+C content was 46.7 mol%. The major cellular fatty acids identified were C, 3-OH-Cand 3-OH-iso-C. Whole-genome comparisons based on average nucleotide identity using blast (ANIb) anddigital DNA-DNA hybridization (dDDH) revealed that the five strains demonstrated ANIb and dDDH values of less than 73.1% and 28.6%, respectively, compared with the type strains of closely related species, such asand. Based on these phenotypic, phylogenetic and genomic data, a novel species of the genus,sp. nov., is proposed, with GTC17253(CCUG77931) as the type strain.