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Peer-reviewed veterinary case report

Spatial Profiling of Germinal Centers in Mouse Secondary Lymphoid Organs Using MACSima Imaging Cyclic Staining Technology.

Year:
2026
Authors:
Molina ISM et al.
Affiliation:
Babraham Institute · United Kingdom

Abstract

We developed a 50-plex imaging protocol using the MACSima platform to characterize the microarchitecture of germinal centers in secondary lymphoid tissues. This workflow combines tissue processing, automated cyclic imaging, image preprocessing, and a new analysis method that detects morphological changes in irregularly shaped stromal cells.

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Original publication: https://europepmc.org/article/MED/41732996