Peer-reviewed veterinary case report
Species-specific PCR assay for the detection of.
- Journal:
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
- Year:
- 2021
- Authors:
- Burgess, Hilary J et al.
- Affiliation:
- Department of Veterinary Pathology · Canada
Abstract
We developed a PCR assay for the detection ofbased on the 18S rRNA gene. Multiple specimens ofwere examined, and the assay consistently produced a small specific PCR product of 306 bp. The PCR assay was also challenged with DNA from 13 otherspecies and 2species, originating from 10 different host species; however, nonspecific DNA amplification and multiple banding patterns were observed, and the amplicon banding patterns varied between different isolates of the same species. Sensitivity was determined to be 6.4 pg of DNA, and an estimated 0.0001% parasitism. This assay can be utilized for species-specific differential detection of.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/34550025/