Spectratype analysis of clonal T cell expansion in murine experimental stroke.

Abstract

T lymphocytes are major contributors to post-ischemic neuroinflammation following ischemic stroke. However, the mode of postischemic T cell activation remains a controversial issue. Therefore, this study aimed to directly investigate antigen-dependent clonal T cell expansion after experimental brain ischemia using spectratype/immunoscope analysis. We detected clonal T cell expansion in ischemic brains and to a lesser extent in secondary lymphatic organs and characterized its time course. This is the first study presenting direct evidence of clonal T cell expansion after stroke - however, with delayed kinetics that make antigen-dependent mechanisms unlikely in acute post-ischemic neuroinflammation.