Peer-reviewed veterinary case report
Real-time PCR test for early detection of acute leptospirosis in dogs
By T.K. Subi et al.·Published in Journal of Veterinary and Animal Sciences·2017·Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy,Thrissur, Kerala- 680 651, IN
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Original publication title: STANDARDISATION OF REAL-TIME PCR FOR THE DETECTION OF ACUTE CANINE LEPTOSPIROSIS
- Species:
- dog
Plain-English summary
A group of dogs suspected of having leptospirosis, a serious infection that can cause fever and other symptoms, were tested using different methods to find the best way to diagnose the disease. The study compared a traditional test called the Microscopic Agglutination Test (MAT) with two types of PCR tests, which are faster and more sensitive. The results showed that the real-time PCR test was more effective than the conventional PCR, allowing for quicker diagnosis and better management of the illness. This means that if your dog shows signs of leptospirosis, a vet might use real-time PCR for a faster and more accurate diagnosis.
People also search for: dog leptospirosis symptoms · how to test for leptospirosis in dogs · dog fever treatment
Abstract
Leptospirosis is one of the most widespread zoonoses. The early diagnosis of the disease based on the symptoms is often difficult because of its overlap ping symptoms with many other febrile illnesses. The present study envisages the evaluation of real time PCR and conventional PCR as compared to the Microscopic Agglutination Test (MAT), for the detection of acute canine leptospirosis. A total of 75 samples were collected from the cases suspected for canine leptospirosis, which include sera for conducting MAT, whole blood for PCR and Real time PCR and urine samples. The sensitivity, specificity and accuracy of conventional and real time PCR assays were determined keeping MAT as the reference. When the sera samples at 1:400 dilution were tested in MAT, 36 of them were found to be positive. The sensitivity, specificity and accuracy of the conventional PCR when compared to MAT was 80.55, 97.4 and 89 percent, respectively. Similarly the values observed for real time PCR vs MAT were 91.6, 84.61 and 88 percent, respectively. From the results, it could be concluded that real time PCR assay standardized in the present study was found to be more sensitive than the conventional PCR. In addition, the real time PCR assay could deliver the results quickly and enable quantification of the organisms in the sample, which would aid in much more efficient case management.
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