Staphylococcus pseudoxylosus sp. nov., isolated from bovine mastitis.

Abstract

Strain S04009, a Gram-stain-positive, coagulase-negative staphylococcus, was isolated from bovine mastitis in France. 16S rRNA gene analysis revealed it to be closely related to the coagulase-negative species Staphylococcusxylosus, Staphylococcussaprophyticus, Staphylococcuscaeli and Staphylococcus edaphicus. At the whole-genome level, strain S04009had an average nucleotide identity value <95&#x200a;%&#x2009;and an inferred DNA-DNA hybridization value <70&#x200a;% when compared to these species. Furthermore, phenotypic characteristics distinguished S04009from those species. From these related species only strain S04009and S. xylosus are able to ferment xylose and these two can be distinguished by the inability of strain S04009to express urease activity. Based on the genotypic and phenotypic results, it is proposed that this isolate is a novel species, with the name Staphylococcus pseudoxylosus sp. nov. The type strain is S04009(=DSM 107950=CCUG 72763=NCTC 14184).