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Peer-reviewed veterinary case report

Strain types of Mycoplasma cynos in dogs with respiratory disease

By Mannering, Sally A et al.·Published in Veterinary microbiology·2009·The Royal Veterinary College, United Kingdom·View original on PubMed

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Original publication title: Strain typing of Mycoplasma cynos isolates from dogs with respiratory disease.

Species:
dog
Dog coughingBreathing & coughDogs

Plain-English summary

A group of dogs with breathing problems was found to have a bacteria called Mycoplasma cynos, which is linked to respiratory disease. Samples were taken from six dogs at two kennels in the UK, and it was discovered that many of the bacteria strains were genetically similar, suggesting they might be spreading within the kennel environment. Some strains were more diverse, indicating that not all infections were the same. This research highlights the importance of understanding how these bacteria can persist and spread among dogs in close quarters.

People also search for: dog breathing problems Mycoplasma cynos · kennel cough treatment · respiratory disease in dogs

Abstract

The association of Mycoplasma cynos with canine infectious respiratory disease is increasingly being recognised. This study describes the strain typing of 14 M. cynos isolates cultured from trachea and bronchoalveolar lavage samples of six dogs with respiratory disease, from two separate kennels in the United Kingdom. The genetic similarity of the isolates was investigated using pulsed-field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD). Most of the isolates from four dogs housed at a re-homing kennel were genetically similar and some isolates from different dogs were indistinguishable by both PFGE and RAPD. These isolates were cultured from dogs with non-overlapping stays in the kennel, which may indicate maintenance of some strains within kennels. A small number of isolates showed much greater genetic heterogeneity and were genetically distinct from the main group of M. cynos strains. There was also a high degree of similarity of the M. cynos type strain (isolated from a dog with respiratory disease in Denmark in 1971) to at least one of the United Kingdom isolates using PFGE analysis, which may suggest possible conservation of pathogenic strains of M. cynos.

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Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/18977617/