Subcapsular Implantation of Pancreatic Islets in Syngeneic, Allogeneic, and Xenogeneic Mice.

Abstract

BACKGROUND: Reliable and reproducible transplantation is essential to the success of a number of particular investigations. Renal subcapsule is the most selected site for islet transplantation mainly owing to its easy access, readiness for retrieval, and possibility of reimplantation. METHODS: Syngeneic, allogeneic, and xenogeneic islets were transplanted into kidney capsules of Balb/C and C57BL/6J mice, and the blood glucose levels of the experimental animals were periodically monitored. Detailed procedures on mouse diabetic model and islet implantation are described. RESULTS: The values of blood glucose measured under varied transplant circumstances are presented, covering syngeneic, allogeneic, and xenogeneic islet transplantations. The methodology is straightforward and has been proven to be practicable and reproducible. CONCLUSIONS: The parallel observations in different transplant situations provide a valuable contribution to and useful information for diabetes-related studies.