subsp.CECT 8145 BPL1Laxative Effects in Loperamide-Induced Constipated SD Rats.

Abstract

Constipation is a common gastrointestinal (GI) state for which probiotics have shown promise as a relief. This study examined the laxative effects of the strainsubsp.CECT 8145 (BPL1) in a loperamide-induced rat model of constipation.Fifty-nine rats were divided into control and loperamide-induced constipation groups. Animals received a 3-day intervention with either placebo or probiotic BPL1at two doses: 1.5 × 10CFU (colony-forming units) (low) and 3 × 10CFU (high). The study assessed several parameters to determine the probiotic's effect, including: stool and gut characteristics, gastrointestinal transit time (GTT), gene expression and gut microbiome composition.: While loperamide significantly decreased stool number, weight and humidity, BPL1supplementation effectively restored these parameters, being more pronounced at a high dose. Microbiome analysis showed that BPL1at a low dose reduced the abundance ofand, associated with constipation. In addition,abundance was negatively correlated with stool humidity. Functional microbiome profiling indicated that BPL1suppressed pathways related to mucin degradation, vancomycin resistance and isoleucine biosynthesis while promoting L-lactate and pyridoxal-P (vitamin B6) biosynthesis, which may support gut motility and barrier integrity.subsp.BPL1exhibits potential as a functional probiotic for relieving constipation through improving stool excretion and consistency, inducing taxonomic changes and beneficial functional modulation of the intestinal microbiome. These findings justify further investigation into the mechanisms of BPL1as a probiotic for constipation management.