Peer-reviewed veterinary case report
Suggested guidelines for immunohistochemical techniques in veterinary diagnostic laboratories.
- Journal:
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
- Year:
- 2008
- Authors:
- Ramos-Vara, José A et al.
- Affiliation:
- Purdue University · United States
Plain-English summary
This document outlines guidelines for using immunohistochemistry (IHC), a laboratory technique that helps diagnose diseases in animals. The guidelines were created by a group of experts to ensure that IHC tests are done consistently and accurately in veterinary labs. They cover important topics like choosing the right antibodies, preparing tissue samples, and ensuring tests are reliable. By following these guidelines, veterinary labs can improve the quality of their diagnostic results, which is crucial for identifying both infections and tumors in pets. Overall, the goal is to make IHC testing more standardized and trustworthy in veterinary medicine.
Abstract
This document is the consensus of the American Association of Veterinary Laboratory Diagnosticians (AAVLD) Subcommittee on Standardization of Immunohistochemistry on a set of guidelines for immunohistochemistry (IHC) testing in veterinary laboratories. Immunohistochemistry is a powerful ancillary methodology frequently used in many veterinary laboratories for both diagnostic and research purposes. However, neither standardization nor validation of IHC tests has been completely achieved in veterinary medicine. This document addresses both issues. Topics covered include antibody selection, fixation, antigen retrieval, antibody incubation, antibody dilutions, tissue and reagent controls, buffers, and detection systems. The validation of an IHC test is addressed for both infectious diseases and neoplastic processes. In addition, storage and handling of IHC reagents, interpretation, quality control and assurance, and troubleshooting are also discussed. Proper standardization and validation of IHC will improve the quality of diagnostics in veterinary laboratories.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/18599844/