Sulfated modification can enhance antiviral activities of Achyranthes bidentata polysaccharide against porcine reproductive and respiratory syndrome virus (PRRSV) in vitro.

Abstract

Achyranthes bidentata polysaccharide (ABPS) was sulfated using the chlorosulfonic acid-pyridine method. The UV-spectrum of sulfated ABPS (sABPS) was characterized with two different absorbance peaks at 196.3 and 262.8 nm. FT-IR spectrum of sABPS exhibited absorption bands at 1236.5 cm(-1) (asymmetrical S=O stretching vibration) and 822.8 cm(-1) (symmetrical C-O-S vibration related to a C-O-SO(3) group). Anti-PRRSV activities of sABPS were tested on MARC-145 cells by MTT method. The results showed that sABPS exhibited significant antiviral activities at lower concentrations in comparison with the non-modified ABPS treated cells, indicating that sulfated modification could enhance antiviral activities of ABPS. These findings may provide the potential value to develop a new-type drug against PRRSV infection.